Domain Of Unknown Function

Recently numerous proteins containing the domain of unknown function duf 3494 were found to bind ice crystals and hence are classified as ibps.

Domain of unknown function. Here we illustrate an integrated genomic enzymology strategy to identify diverse functions within the duf1537 family pf07005. These families have been collected together in the pfam database using the prefix duf followed by a number with examples being duf2992 and duf1220 there are now over 3 000 duf families within the pfam database representing over 20 of known families. Although the 3d structure is always a discontinuous β solenoid with a triangular cross section and an adjacent alpha.

A penicillin binding domain and two domains of unknown functions figure 771 1. The latter two domains are inserted in the penicillin binding domain between the sxxk and sxn motifs in the way of matryoshka dolls. Given their biological importance large scale strategies are required to accomplish their functional assignments.

Domain of unknown function duf families constitute 3 892 of the 16 295 families in the pfam database release 29 0. A large number of genes with duf domain of unknown function from different species have been deposited in genbank. For example duf1943 has been found encoded by the hypothetical protein genes of dro.

They can be found in different organisms including bacteria yeasts and microalgae supporting the hypothesis of horizontal transfer of its gene. Recombinant duf959 produced in mammalian cells consisted of 50 glycans and had a molecular mass of 63 kda. A domain of unknown function duf is a protein domain that has no characterised function.

Mutations in duf288 proteins cause cellulose deficiency in both primary and secondary cell wall. This is surprising as a large number of dufs are shared between bacteria and other domains of life. These duf domains are either encoded by genes of known function or by genes of unknown function.

Domains of unknown function occur in large numbers in all kingdoms of life ranging from about just over 1 500 in eukaryotes to 2 704 in bacteria see table s1b and fig. The penicillin binding domain shares its overall fold with other penicillin binding proteins and the serine β lactamases. However duf288 proteins do not affect csc assembly suggesting that duf288 proteins might not directly mediate cellulose.